Proteins are made up of a series of linked amino acids. The makeup of each protein dictates its structure, which then determines its function. Peptides are a short series of amino acid residues that are linked together by peptide bonds. Short peptides have been shown to play an important role in regulating gene expression, and in restoring the damaging genetic changes that occur with age.
Peptide therapy aims to renew the strength of signals received by cells to either induce peptide production or renew normal signaling processes, thereby rejuvenating and revitalizing tissues as well as the organism as a whole.
At Top Pet Pro, we believe that better health is achievable through safe, scientifically tested organopeptides. Top Pet Pro is an R&D biotech company, a subsidiary of European Wellness Biomedical Group (EW) that is focused on the scientific study of existing products manufactured by European Wellness Biomedical Group, as well as developing innovative new products in collaboration with a Colorado-based US FDA registered facility.
Our organopeptides, which are manufactured by European Wellness at their manufacturing facility in Europe, include Nano Organo Peptides (NOPs) and Mito Organelles (MO) Peptides, which are extracted from mammalian stem cells and are processed through a proprietary parallel-extraction process that includes multiple ultrafiltrate steps through specialized millipores to obtain the cellular material within the cell, known as the molecular-level ultrafiltrates.
We are committed to ensuring that all organopeptide products are pathogen-free and safe. These organopeptides are being used rejuvenate and improve tissue that is damaged by disease or aging.
Protein characterization using mass spectrometry
Despite the numerous studies highlighting the therapeutic effectiveness of NOP and MO peptides and the established procedures documented on obtaining them, little is known of the exact makeup of these formulations.
Mass spectrometry (MS) has been shown to identify and quantify these peptides and is thought to be able to identify the peptides derived from peptides obtained from cells. By searching the experimentally derived peaks against a database of known proteins, peptides or molecules, it is possible to identify the peptides. MS is a low cost and high-volume technology commonly used to identify the components of unknown solutions and characterise proteins. MALDI-TOF utilizes a protein fingerprinting method in which the sample is digested by an enzyme that can be searched against existing databases to identify it.
In a recently published study in Clinical Medicine Insights [1] here, our scientists have outlined the experimental methods and the results from performing mass spectroscopy analysis on EW peptides to characterise them. Protein characterization uses experimental methods to identify and understand each protein. It uses experimental methods to detect and isolate each protein and purify it to characterize its structure and function.
The paper outlines the experimental methods and the results from performing MS on various peptides from the company European Wellness (EW). The results of our study using MALDI-TOF to analyze the MO sample indicated that there were five major peptide products of interest in batch 1 and six major peptide products in batch 2, and their masses.
The experimentally derived peaks were searched against a database of known proteins to identify the peptides present in each sample, as shown below:
Source
Good, A.., Wells, A. ., Katz, B. ., Alexander, M. ., Klokol, D. ., Chen, M. K., Wong, M. B. ., Cox, D. C. ., & Lakey, J. R. . (2022). MALDI-ToF Analysis of Mitochondrial Peptides. Clinical Medicine Insights, 3(2), 297–303. https://doi.org/10.52845/CMI/2022-3-2-3
It’s important to note that slight differences in peptide products between batches is likely due to the heterogeneous nature of cellularly-derived solutions and differences that occurred during the extraction process, and as a result, further research must be conducted to replicate these results.
The next step is to undertake further research to confirm the identity of these peptides and discover their significance in peptide therapy.
References
Good, A.., Wells, A. ., Katz, B. ., Alexander, M. ., Klokol, D. ., Chen, M. K., Wong, M. B. ., Cox, D. C. ., & Lakey, J. R. . (2022). MALDI-ToF Analysis of Mitochondrial Peptides. Clinical Medicine Insights, 3(2), 297–303. https://doi.org/10.52845/CMI/2022-3-2-3
